LOCUS       40924_4729        5287 bp DNA     circular SYN 18-DEC-2018
DEFINITION  S. pombe expression vector pARG1B DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     A novel series of vectors for chromosomal integration in fission 
            yeast
  JOURNAL   Biochem. Biophys. Res. Commun. 374 (2), 315-319 (2008)
  PUBMED    18634753
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Fission yeast integration vector pARG1B
  JOURNAL   Published Only in Database (2007)
REFERENCE   3  (bases 1 to 5287)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical 
            Genetics Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL
            :http://cgl.riken.go.jp/
REFERENCE   4  (bases 1 to 5287)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5287)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem. 
            Biophys. Res. Commun."; date: "2008"; volume: "374"; issue: "2"; 
            pages: "315-319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Published 
            Only in Database (2007)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical Genetics 
            Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL 
            :http://cgl.riken.go.jp/"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     Fission yeast expression vector.
FEATURES             Location/Qualifiers
     source          1..5287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      21..208
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    241..894
                     /pseudo
                     /gene="arg1"
                     /label=derived from Schizosaccharomyces pombe
                     /note="derived from Schizosaccharomyces pombe"
     CDS             241..894
                     /pseudo
                     /codon_start=1
                     /gene="arg1"
                     /label=arg1
                     /note="NotI site is created at the middle of this fragment 
                     (551-558)."
                     /translation="SRSC*R*GLLFV*QGRS*IH*LHLWRSCYIFGSCSS*SC*TCR*S
                     MLQISP**QPFLQRACH*AI*CH**FFGKK*RYCWPHKNFLCQLWY*GQ*DSLEVRS*G
                     GRV*EVWRREESNCLL**LFPRPFFGIS*YHCEPQI*TWFPTITS*CRPSRL**PCIHR
                     AICQ*QNCGCYCRARSR*RWYLSCQA*IFDCFAQGL**GWCFFNL**NSMRTWSQ"
     gene            241..894
                     /pseudo
                     /gene="arg1"
                     /label=arg1
     promoter        902..1309
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        1316..1363
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             1382..1777
                     /codon_start=1
                     /label=BSD
                     /note="blasticidin S deaminase"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     terminator      1874..2121
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(2147..2163)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2376..2831
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3113..3217
                     /label=AmpR promoter
     CDS             3218..4075
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4249..4837
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5125..5146
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5161..5191
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5199..5215
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5223..5239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag
       61 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
      121 tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagtatgagg
      181 tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg gtaatatact
      241 agtcgtagct gctaaaggtg agggctccta tttgtttgac aaggaaggtc gtaaatacat
      301 tgacttcacc tctggcgtag ctgttacatc tttgggtcat gctcatcctg aagttgctag
      361 acttgccgct gatcaatgct ccaaattagt ccatagtagc aaccttttct acaacgagcc
      421 tgccattgag ctatctaatg tcattaataa ttctttggca aaaaatagcg gtattgctgg
      481 ccccacaaaa attttctttg ccaattgtgg tactgaggcc aatgagacag ccttgaagtt
      541 cgctcgtaag gcggccgcgt ttgagaagta tggagaaggg aagagtcaaa ttgtttactt
      601 taataactct ttccacggcc gttctttggg atctcttagt atcactgcga accccaaata
      661 taaacgtggt ttccaaccat tacttcctga tgtcgtccaa gccgtctata atgaccctgc
      721 atccatagag caatttgtca atgacaaaac tgcggctgtt attgtcgagc ccgttcaagg
      781 tgaaggtggt atttgtcctg ccaagcctga atttttgatt gctttgcgca aggcctgtga
      841 taaggttggt gcttctttaa tctatgatga aattcaatgc ggacttggtc gcagatctag
      901 cccacacacc atagcttcaa aatgtttcta ctcctttttt actcttccag attttctcgg
      961 actccgcgca tcgccgtacc acttcaaaac acccaagcac agcatactaa atttcccctc
     1021 tttcttcctc tagggtgtcg ttaattaccc gtactaaagg tttggaaaag aaaaaagaga
     1081 ccgcctcgtt tctttttctt cgtcgaaaaa ggcaataaaa atttttatca cgtttctttt
     1141 tcttgaaaat tttttttttt agtttttttc tctttcgatg acctcccatt gatatttaag
     1201 ttaataaacg gtcttcaatt tctcaagttt cagtttcatt tttcttgttc tattacaact
     1261 ttttttactt cttgctcatt agaaagaaag catagcaatc taatctaagg gcggcgttga
     1321 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
     1381 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
     1441 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
     1501 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
     1561 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
     1621 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
     1681 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
     1741 tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gcacttcgtg gccgaggagc
     1801 aggactgaca cgtccgacgc ggcccgacgg gtccgaggcc tcggagatcc gtcccccttt
     1861 tcctttgtcg atatcatgta attagttatg tcacgcttac attcacgccc tccccccaca
     1921 tccgctctaa ccgaaaagga aggagttaga caacctgaag tctaggtccc tatttatttt
     1981 tttatagtta tgttagtatt aagaacgtta tttatatttc aaatttttct tttttttctg
     2041 tacagacgcg tgtacgcatg taacattata ctgaaaacct tgcttgagaa ggttttggga
     2101 cgctcgaagg ctttaatttg caagctgttt aaacgagctc gaattcactg gccgtcgttt
     2161 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     2221 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     2281 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
     2341 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
     2401 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     2461 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     2521 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     2581 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     2641 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     2701 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggccta
     2761 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     2821 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     2881 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     2941 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     3001 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     3061 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     3121 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     3181 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     3241 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     3301 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     3361 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     3421 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     3481 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     3541 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     3601 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     3661 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     3721 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     3781 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     3841 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     3901 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     3961 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     4021 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     4081 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     4141 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     4201 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4261 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4321 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4381 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4441 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4501 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4561 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     4621 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4681 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4741 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4801 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     4861 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     4921 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     4981 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     5041 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     5101 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     5161 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     5221 cacaggaaac agctatgacc atgattacgc caagcttgca tgcctgcagg tcgactctag
     5281 aggatcc
//
